By Curtis A. Machida
Oregon well-being Sciences Univ., Beaverton. offers a set of reproducible tools for the learn of regulators of CNS-mediated habit and neural functionality. For neurobiologists, neurochemists, neurologists, and pharmacologists. DNLM: Receptors, Adrenergic - research.
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Moore, D. , Seidman, J. , Smith, J. , et al. (1994) Differential display of mRNA by PCR, in Current Protocols in Molecular Biology, vol. 2, Wiley, Interscience, New York, pp. 8. 44. , Kingston, R. , Moore, D. , Seidman, J. , Smith, J. , et al. (1998) Preparation of genomic DNA from mammalian tissue, in Current Protocols in Molecular Biology, vol. 1, Wiley, Interscience, New York, pp. 3. 45. Pierce, J. C. and Sternberg, N. L. (1992) Using Bacteriophage P1 system to clone high molecular weight genomic DNA, in Methods in Enzymology, vol.
Species or on all the possible nucleotide sequences for the amino acids at the primer site. However, the number of degenerate bases should be kept to a minimum. In particular, the base composition at the 3′ end should be conserved among all the species or subtypes (see Note 19). 4. Reverse Transcription and the Selection of Primers Several different types of primers and reverse transcriptase (RT) enzymes can be used to form the first-strand or cDNA from either total or poly A+ RNA templates (see Notes 20 and 21).
1995) α- and β-adrenoceptors: From the gene to the clinic. 2. Structure–activity relationships and therapeutic applications. J. Med. Chem. 38, 3681–3716. 32 Scofield, Deupree, and Bylund 3. Ahlquist, R. P. (1948) A study of adrenotropic receptors. Am. J. Physiol. 153, 586–600. 4. Bylund, D. B. (1988) Subtypes of α 2-adrenoceptors: pharmacological and molecular biological evidence converge. Trends Pharmacol. Sci. 9, 356–361. 5. Bylund, D. , Eikenberg, D. , Hieble, J. , Langer, S. , Lefkowitz, R.
Adrenergic Receptor Protocols by Curtis A. Machida